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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NBP1-49536 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49536, RRID:AB_10011589
- Product name
- Rabbit Polyclonal Pyruvate Carboxylase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Generation of Human Pyruvate Carboxylase Knockout Cell Lines Using Retrovirus Expressing Short Hairpin RNA and CRISPR-Cas9 as Models to Study Its Metabolic Role in Cancer Research.
Autophagy Regulates the Liver Clock and Glucose Metabolism by Degrading CRY1.
Mitochondrial pyruvate carrier function determines cell stemness and metabolic reprogramming in cancer cells.
Diet-induced obesity in the selenocysteine lyase knockout mouse.
Niclosamide ethanolamine-induced mild mitochondrial uncoupling improves diabetic symptoms in mice.
Toxicity generated through inhibition of pyruvate carboxylase and carnitine palmitoyl transferase-1 is similar to high glucose/palmitate-induced glucolipotoxicity in INS-1 beta cells.
Enhanced Ca(2+)-dependent glutamate release from astrocytes of the BACHD Huntington's disease mouse model.
Divergent effects of human cytomegalovirus and herpes simplex virus-1 on cellular metabolism.
Rattanapornsompong K, Ngamkham J, Chavalit T, Jitrapakdee S
Methods in molecular biology (Clifton, N.J.) 2019;1916:273-288
Methods in molecular biology (Clifton, N.J.) 2019;1916:273-288
Autophagy Regulates the Liver Clock and Glucose Metabolism by Degrading CRY1.
Toledo M, Batista-Gonzalez A, Merheb E, Aoun ML, Tarabra E, Feng D, Sarparanta J, Merlo P, Botrè F, Schwartz GJ, Pessin JE, Singh R
Cell metabolism 2018 Aug 7;28(2):268-281.e4
Cell metabolism 2018 Aug 7;28(2):268-281.e4
Mitochondrial pyruvate carrier function determines cell stemness and metabolic reprogramming in cancer cells.
Li X, Han G, Li X, Kan Q, Fan Z, Li Y, Ji Y, Zhao J, Zhang M, Grigalavicius M, Berge V, Goscinski MA, Nesland JM, Suo Z
Oncotarget 2017 Jul 11;8(28):46363-46380
Oncotarget 2017 Jul 11;8(28):46363-46380
Diet-induced obesity in the selenocysteine lyase knockout mouse.
Seale LA, Gilman CL, Hashimoto AC, Ogawa-Wong AN, Berry MJ
Antioxidants & redox signaling 2015 Oct 1;23(10):761-74
Antioxidants & redox signaling 2015 Oct 1;23(10):761-74
Niclosamide ethanolamine-induced mild mitochondrial uncoupling improves diabetic symptoms in mice.
Tao H, Zhang Y, Zeng X, Shulman GI, Jin S
Nature medicine 2014 Nov;20(11):1263-9
Nature medicine 2014 Nov;20(11):1263-9
Toxicity generated through inhibition of pyruvate carboxylase and carnitine palmitoyl transferase-1 is similar to high glucose/palmitate-induced glucolipotoxicity in INS-1 beta cells.
Lee JH, Jung IR, Choi SE, Lee SM, Lee SJ, Han SJ, Kim HJ, Kim DJ, Lee KW, Kang Y
Molecular and cellular endocrinology 2014 Mar 5;383(1-2):48-59
Molecular and cellular endocrinology 2014 Mar 5;383(1-2):48-59
Enhanced Ca(2+)-dependent glutamate release from astrocytes of the BACHD Huntington's disease mouse model.
Lee W, Reyes RC, Gottipati MK, Lewis K, Lesort M, Parpura V, Gray M
Neurobiology of disease 2013 Oct;58:192-9
Neurobiology of disease 2013 Oct;58:192-9
Divergent effects of human cytomegalovirus and herpes simplex virus-1 on cellular metabolism.
Vastag L, Koyuncu E, Grady SL, Shenk TE, Rabinowitz JD
PLoS pathogens 2011 Jul;7(7):e1002124
PLoS pathogens 2011 Jul;7(7):e1002124
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pyruvate Carboxylase Antibody [NBP1-49536] - Analysis of Pyruvate Carboxylase in A. Human liver extracts and B. Mouse liver extracts
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP1-49536] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse kidney using rabbit anti-Pyruvate Carboxylase antibody at 1:200 dilution. The signal was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The nuclei were counterstained using hematoxylin. This Pyruvate Carboxylase antibody generated a diffused cytoplasmic staining in all the tubules and a subset of cells in glomerular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP1-49536] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse adipose tissue using rabbit anti-Pyruvate Carboxylase antibody at 1:200 dilution. The signal was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The nuclei were counterstained using hematoxylin. This Pyruvate Carboxylase antibody generated a specific staining of the fat cells in the tested section.
