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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB27672 - Provider product page
- Provider
- Abnova Corporation
- Product name
- Znrf1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of Znrf1.
- Antigen sequence
SDSTYAHGNGYQET- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Lane 1: N2a mock transfection.Lane 2: N2a overexpressing mouse Znrf1 and probed with Znrf1 polycolnal antibody (Cat # PAB27672) (1ug/ml).Lane 3: Znrf1 polycolnal antibody (Cat # PAB27672) staining of mouse brain lysates and Lane 4: adult cerebellum. Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Znrf1 polycolnal antibody (Cat # PAB27672) (10ug/ml) staining of paraffin embedded mouse cerebral cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1:100).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
