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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- A-104 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Ubiquitin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. This antibody detects human Ubiquitin and Ubiquitin+1 in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 50 ug
- Storage
- Store the unopened product at -20 degreesC. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 degreesC is not recommended. Do not use past expiration date.
Submitted references Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase.
Trichostatin A, a histone deacetylase inhibitor suppresses NADPH Oxidase 4-Derived Redox Signalling and Angiogenesis.
Fottner M, Brunner AD, Bittl V, Horn-Ghetko D, Jussupow A, Kaila VRI, Bremm A, Lang K
Nature chemical biology 2019 Mar;15(3):276-284
Nature chemical biology 2019 Mar;15(3):276-284
Trichostatin A, a histone deacetylase inhibitor suppresses NADPH Oxidase 4-Derived Redox Signalling and Angiogenesis.
Hakami NY, Dusting GJ, Peshavariya HM
Journal of cellular and molecular medicine 2016 Oct;20(10):1932-44
Journal of cellular and molecular medicine 2016 Oct;20(10):1932-44
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Ubiquitin by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Ubiquitin Monoclonal Antibody (Catalog # A-104) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Ubiquitin at approximately 5-10 & 37-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
