Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA501984 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501984, RRID:AB_11124773
- Product name
- SPR mouse monoclonal antibody, clone OTI1F4 (formerly 1F4)
- Antibody type
- Monoclonal
- Description
- SPR mouse monoclonal antibody, clone OTI1F4 (formerly 1F4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SPR
- Isotype
- IgG
- Antibody clone number
- OTI1F4
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (RC205679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SPR mouse monoclonal antibody (TA501984) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR(RC205679).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC205679 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SPR antibody(TA501984), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-SPR antibody(TA501984),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
