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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-22293 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SPR Polyclonal Antibody (Product # PA5-22293). Sample (30 µg of whole cell lysate). Lane A: A431. 12% SDS PAGE. SPR Polyclonal Antibody (Product # PA5-22293) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SPR in methanol-fixed HeLa cells using a SPR polyclonal antibody (Product # PA5-22293) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SPR Polyclonal Antibody detects SPR protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SPR Polyclonal Antibody (Product # PA5-22293) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SPR Polyclonal Antibody immunoprecipitates SPR protein in IP experiments. IP samples: A431 whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of SPR protein by 4 µg SPR Polyclonal Antibody (Product # PA5-22293). 5 % SDS-PAGE. The immunoprecipitated SPR protein was detected by SPR Polyclonal Antibody (Product # PA5-22293) diluted at 1:500.
