Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP09318PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09318PU-N, RRID:AB_2035654
- Product name
- anti PPP1R13L (780-797)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to amino acids 780-797 of Human iASPP (isoform 1) protein
- Reactivity
- Human, Mouse, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.19 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using AP09318PU-N iASPP antibody shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1/1,500 in 5% LOTTO/PBS overnight at 4°C. The membrane was washed and reacted with a 1/10,000 dilution of IRDye(TM)800 conjugated Goat anti-Rabbit IgG [H&L] for 45 min at RT (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP09318PU-N iASPP antibody staining of Formalin-Fixed, Paraffin Embedded Human heart tissue. This antibody shows strong cytoplasmic and membranous staining of myocytes. Brown color indicates presence of protein, blue color shows cell nuclei.