PA5-31148

antibody from Invitrogen Antibodies

Targeting: SMNDC1 SMNR, SPF30, TDRD16C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31148

Provider

Invitrogen Antibodies

Product name

SMNDC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SMNDC1 using 30 µg of IMR32 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a SMNDC1 polyclonal antibody (Product # PA5-31148) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SMNDC1 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SMNDC1 Polyclonal Antibody (Product # PA5-31148) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SMNDC1 in paraformaldehyde-fixed MCF-7 cells using a SMNDC1 polyclonal antibody (Product # PA5-31148) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SMNDC1 (Product # PA5-31148) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SMNDC1 Polyclonal Antibody detects SMNDC1 protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SMNDC1 Polyclonal Antibody (Product # PA5-31148) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SNU1 xenograft, using SMNDC1 (Product # PA5-31148) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.