TA327941

antibody from OriGene

Targeting: H2AX H2AFX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA327941

Provider

OriGene

Product name

Mouse Monoclonal anti-H2A.X Phospho (Ser139) Antibody

Antibody type

Monoclonal

Description

Mouse Monoclonal anti-H2A.X Phospho (Ser139) Antibody

Host

Mouse

Conjugate

Unconjugated

Epitope

H2AFX

Isotype

IgG

Antibody clone number

2F3

Vial size

100 µg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract, untreated (Lane 3) and UV treated (Lane 4) Jurkat cell extract were western blotted using anti-phospho-H2A.X (Ser 139) antibody, clone: 2F3.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.

Validation comment

IF