Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 613410 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#613410, RRID:AB_2616774
- Product name
- Alexa Fluor® 594 anti-H2A.X-Phosphorylated (Ser139)
- Antibody type
- Monoclonal
- Antigen
- Modified peptide
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 2F3
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of Alexa Fluor® 594 anti-H2A.X Phospho (Ser139) (clone 2F3) (red) in 5% FBS overnight followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
- Conjugate
- Yellow dye