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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ABIN256363 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-H2A Histone Family, Member X (H2AFX) (N-Term) antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term
- Isotype
- IgG
- Vial size
- 0.2 mL
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
- Handling
- Avoid freeze-thaw cycles
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Hela cells were fixed in 4% paraformaldehyde , washed in PBS, and permeabilized using 0.5% Triton X-100 for 5 minutes. Cells were then incubated with a 1:50 dilution of H2A.X antibody (Poly6133) for 1 hr, washed twice using 0.1% Triton X-100 in PBS and incubated with goat anti-rabbit conjugated to Alexa Fluor® 488. After washing twice, cells were mounted and examined on a fluorescence microscope. 100 X magnification.
