Explore
Validate
Learn
Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 613402 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#613402, RRID:AB_315795
- Product name
- Purified anti-H2A.X Phospho (Ser139)
- Antibody type
- Monoclonal
- Antigen
- Modified peptide
- Description
- H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting and immunofluorescence.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store at 4° C.
Submitted references Effect of Antioxidants on X-ray-induced γ-H2AX Foci in Human Blood Lymphocytes: Preliminary Observations.
Blockade of TGF-β signaling by the TGFβR-I kinase inhibitor LY2109761 enhances radiation response and prolongs survival in glioblastoma.
MRE11 and COM1/SAE2 are required for double-strand break repair and efficient chromosome pairing during meiosis of the protist Tetrahymena.
RUNX3 modulates DNA damage-mediated phosphorylation of tumor suppressor p53 at Ser-15 and acts as a co-activator for p53.
A novel, drug-based, cellular assay for the activity of neurotoxic mutants of the prion protein.
NFBD1/MDC1 participates in the regulation of G2/M transition in mammalian cells.
A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks.
gamma-H2AX foci as a biomarker for patient X-ray exposure in pediatric cardiac catheterization: are we underestimating radiation risks?
Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage.
DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena.
Differential roles of telomere attrition in type I and II endometrial carcinogenesis.
Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age.
Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis.
Kuefner MA, Brand M, Ehrlich J, Braga L, Uder M, Semelka RC
Radiology 2012 Jul;264(1):59-67
Radiology 2012 Jul;264(1):59-67
Blockade of TGF-β signaling by the TGFβR-I kinase inhibitor LY2109761 enhances radiation response and prolongs survival in glioblastoma.
Zhang M, Kleber S, Röhrich M, Timke C, Han N, Tuettenberg J, Martin-Villalba A, Debus J, Peschke P, Wirkner U, Lahn M, Huber PE
Cancer research 2011 Dec 1;71(23):7155-67
Cancer research 2011 Dec 1;71(23):7155-67
MRE11 and COM1/SAE2 are required for double-strand break repair and efficient chromosome pairing during meiosis of the protist Tetrahymena.
Lukaszewicz A, Howard-Till RA, Novatchkova M, Mochizuki K, Loidl J
Chromosoma 2010 Oct;119(5):505-18
Chromosoma 2010 Oct;119(5):505-18
RUNX3 modulates DNA damage-mediated phosphorylation of tumor suppressor p53 at Ser-15 and acts as a co-activator for p53.
Yamada C, Ozaki T, Ando K, Suenaga Y, Inoue K, Ito Y, Okoshi R, Kageyama H, Kimura H, Miyazaki M, Nakagawara A
The Journal of biological chemistry 2010 May 28;285(22):16693-703
The Journal of biological chemistry 2010 May 28;285(22):16693-703
A novel, drug-based, cellular assay for the activity of neurotoxic mutants of the prion protein.
Massignan T, Stewart RS, Biasini E, Solomon IH, Bonetto V, Chiesa R, Harris DA
The Journal of biological chemistry 2010 Mar 5;285(10):7752-65
The Journal of biological chemistry 2010 Mar 5;285(10):7752-65
NFBD1/MDC1 participates in the regulation of G2/M transition in mammalian cells.
Bu Y, Suenaga Y, Okoshi R, Sang M, Kubo N, Song F, Nakagawara A, Ozaki T
Biochemical and biophysical research communications 2010 Jun 25;397(2):157-62
Biochemical and biophysical research communications 2010 Jun 25;397(2):157-62
A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks.
Suzuki K, Yamauchi M, Oka Y, Suzuki M, Yamashita S
Nucleic acids research 2010 Jul;38(12):e129
Nucleic acids research 2010 Jul;38(12):e129
gamma-H2AX foci as a biomarker for patient X-ray exposure in pediatric cardiac catheterization: are we underestimating radiation risks?
Beels L, Bacher K, De Wolf D, Werbrouck J, Thierens H
Circulation 2009 Nov 10;120(19):1903-9
Circulation 2009 Nov 10;120(19):1903-9
Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage.
Loidl J, Mochizuki K
Molecular biology of the cell 2009 May;20(9):2428-37
Molecular biology of the cell 2009 May;20(9):2428-37
DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena.
Mochizuki K, Novatchkova M, Loidl J
Journal of cell science 2008 Jul 1;121(Pt 13):2148-58
Journal of cell science 2008 Jul 1;121(Pt 13):2148-58
Differential roles of telomere attrition in type I and II endometrial carcinogenesis.
Akbay EA, Contreras CM, Perera SA, Sullivan JP, Broaddus RR, Schorge JO, Ashfaq R, Saboorian H, Wong KK, Castrillon DH
The American journal of pathology 2008 Aug;173(2):536-44
The American journal of pathology 2008 Aug;173(2):536-44
Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age.
Rossi DJ, Bryder D, Seita J, Nussenzweig A, Hoeijmakers J, Weissman IL
Nature 2007 Jun 7;447(7145):725-9
Nature 2007 Jun 7;447(7145):725-9
Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis.
Xiao R, Sun Y, Ding JH, Lin S, Rose DW, Rosenfeld MG, Fu XD, Li X
Molecular and cellular biology 2007 Aug;27(15):5393-402
Molecular and cellular biology 2007 Aug;27(15):5393-402
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
- Conjugate
- Unconjugated
