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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [21]
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- Validations
- Western blot [2]
- Immunohistochemistry [6]
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- Product number
- NB100-2280 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2280, RRID:AB_10000580
- Product name
- Rabbit Polyclonal gamma H2AX Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Inhibition of thyroid hormone signaling protects retinal pigment epithelium and photoreceptors from cell death in a mouse model of age-related macular degeneration.
Resveratrol attenuates doxorubicin-induced meiotic failure through inhibiting oxidative stress and apoptosis in mouse oocytes.
p73 regulates epidermal wound healing and induced keratinocyte programming.
Oncogenic Akt-FOXO3 loop favors tumor-promoting modes and enhances oxidative damage-associated hepatocellular carcinogenesis.
The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8.
E2F1 proteolysis via SCF-cyclin F underlies synthetic lethality between cyclin F loss and Chk1 inhibition.
GSK-3β protects fetal oocytes from premature death via modulating TAp63 expression in mice.
Establishment and characterization of a canine soft tissue sarcoma patient-derived xenograft model.
Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression.
Lurbinectedin induces depletion of tumor-associated macrophages, an essential component of its in vivo synergism with gemcitabine, in pancreatic adenocarcinoma mouse models.
The polarity protein Scrib mediates epidermal development and exerts a tumor suppressive function during skin carcinogenesis.
Fibulin-5 Blocks Microenvironmental ROS in Pancreatic Cancer.
Increased expression of stathmin and elongation factor 1α in precancerous nodules with telomere dysfunction in hepatitis B viral cirrhotic patients.
PinX1, a telomere repeat-binding factor 1 (TRF1)-interacting protein, maintains telomere integrity by modulating TRF1 homeostasis, the process in which human telomerase reverse Transcriptase (hTERT) plays dual roles.
Wee1 inhibition by MK-1775 leads to tumor inhibition and enhances efficacy of gemcitabine in human sarcomas.
E2F7 represses a network of oscillating cell cycle genes to control S-phase progression.
Mutant telomerase RNAs induce DNA damage and apoptosis via the TRF2-ATM pathway in telomerase-overexpressing primary fibroblasts.
Loss of fibulin-5 binding to beta1 integrins inhibits tumor growth by increasing the level of ROS.
Chemopreventive agent 3,3'-diindolylmethane selectively induces proteasomal degradation of class I histone deacetylases.
Potentiation of temozolomide cytotoxicity by inhibition of DNA polymerase beta is accentuated by BRCA2 mutation.
Dual phosphoinositide 3-kinase/mammalian target of rapamycin blockade is an effective radiosensitizing strategy for the treatment of non-small cell lung cancer harboring K-RAS mutations.
Ma H, Yang F, Ding XQ
Cell death & disease 2020 Jan 13;11(1):24
Cell death & disease 2020 Jan 13;11(1):24
Resveratrol attenuates doxorubicin-induced meiotic failure through inhibiting oxidative stress and apoptosis in mouse oocytes.
Han J, Wang H, Zhang T, Chen Z, Zhao T, Lin L, Xia G, Wang C
Aging 2020 Apr 30;12(9):7717-7728
Aging 2020 Apr 30;12(9):7717-7728
p73 regulates epidermal wound healing and induced keratinocyte programming.
Beeler JS, Marshall CB, Gonzalez-Ericsson PI, Shaver TM, Santos Guasch GL, Lea ST, Johnson KN, Jin H, Venters BJ, Sanders ME, Pietenpol JA
PloS one 2019;14(6):e0218458
PloS one 2019;14(6):e0218458
Oncogenic Akt-FOXO3 loop favors tumor-promoting modes and enhances oxidative damage-associated hepatocellular carcinogenesis.
Lu M, Hartmann D, Braren R, Gupta A, Wang B, Wang Y, Mogler C, Cheng Z, Wirth T, Friess H, Kleeff J, Hüser N, Sunami Y
BMC cancer 2019 Sep 5;19(1):887
BMC cancer 2019 Sep 5;19(1):887
The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8.
Singh AN, Oehler J, Torrecilla I, Kilgas S, Li S, Vaz B, Guérillon C, Fielden J, Hernandez-Carralero E, Cabrera E, Tullis ID, Meerang M, Barber PR, Freire R, Parsons J, Vojnovic B, Kiltie AE, Mailand N, Ramadan K
The EMBO journal 2019 Oct 4;38(21):e102361
The EMBO journal 2019 Oct 4;38(21):e102361
E2F1 proteolysis via SCF-cyclin F underlies synthetic lethality between cyclin F loss and Chk1 inhibition.
Burdova K, Yang H, Faedda R, Hume S, Chauhan J, Ebner D, Kessler BM, Vendrell I, Drewry DH, Wells CI, Hatch SB, Dianov GL, Buffa FM, D'Angiolella V
The EMBO journal 2019 Oct 15;38(20):e101443
The EMBO journal 2019 Oct 15;38(20):e101443
GSK-3β protects fetal oocytes from premature death via modulating TAp63 expression in mice.
Wen J, Yan H, He M, Zhang T, Mu X, Wang H, Zhang H, Xia G, Wang C
BMC biology 2019 Mar 12;17(1):23
BMC biology 2019 Mar 12;17(1):23
Establishment and characterization of a canine soft tissue sarcoma patient-derived xenograft model.
Frazier JP, Beirne E, Ditzler SH, Tretyak I, Casalini JR, Thirstrup DJ, Knoblaugh S, Ward JG, Tripp CD, Klinghoffer RA
Veterinary and comparative oncology 2017 Sep;15(3):754-763
Veterinary and comparative oncology 2017 Sep;15(3):754-763
Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression.
Franz A, Pirson PA, Pilger D, Halder S, Achuthankutty D, Kashkar H, Ramadan K, Hoppe T
Nature communications 2016 Feb 4;7:10612
Nature communications 2016 Feb 4;7:10612
Lurbinectedin induces depletion of tumor-associated macrophages, an essential component of its in vivo synergism with gemcitabine, in pancreatic adenocarcinoma mouse models.
Céspedes MV, Guillén MJ, López-Casas PP, Sarno F, Gallardo A, Álamo P, Cuevas C, Hidalgo M, Galmarini CM, Allavena P, Avilés P, Mangues R
Disease models & mechanisms 2016 Dec 1;9(12):1461-1471
Disease models & mechanisms 2016 Dec 1;9(12):1461-1471
The polarity protein Scrib mediates epidermal development and exerts a tumor suppressive function during skin carcinogenesis.
Pearson HB, McGlinn E, Phesse TJ, Schlüter H, Srikumar A, Gödde NJ, Woelwer CB, Ryan A, Phillips WA, Ernst M, Kaur P, Humbert P
Molecular cancer 2015 Sep 17;14:169
Molecular cancer 2015 Sep 17;14:169
Fibulin-5 Blocks Microenvironmental ROS in Pancreatic Cancer.
Wang M, Topalovski M, Toombs JE, Wright CM, Moore ZR, Boothman DA, Yanagisawa H, Wang H, Witkiewicz A, Castrillon DH, Brekken RA
Cancer research 2015 Dec 1;75(23):5058-69
Cancer research 2015 Dec 1;75(23):5058-69
Increased expression of stathmin and elongation factor 1α in precancerous nodules with telomere dysfunction in hepatitis B viral cirrhotic patients.
Ahn EY, Yoo JE, Rhee H, Kim MS, Choi J, Ko JE, Lee JS, Park YN
Journal of translational medicine 2014 May 31;12:154
Journal of translational medicine 2014 May 31;12:154
PinX1, a telomere repeat-binding factor 1 (TRF1)-interacting protein, maintains telomere integrity by modulating TRF1 homeostasis, the process in which human telomerase reverse Transcriptase (hTERT) plays dual roles.
Yoo JE, Park YN, Oh BK
The Journal of biological chemistry 2014 Mar 7;289(10):6886-6898
The Journal of biological chemistry 2014 Mar 7;289(10):6886-6898
Wee1 inhibition by MK-1775 leads to tumor inhibition and enhances efficacy of gemcitabine in human sarcomas.
Kreahling JM, Foroutan P, Reed D, Martinez G, Razabdouski T, Bui MM, Raghavan M, Letson D, Gillies RJ, Altiok S
PloS one 2013;8(3):e57523
PloS one 2013;8(3):e57523
E2F7 represses a network of oscillating cell cycle genes to control S-phase progression.
Westendorp B, Mokry M, Groot Koerkamp MJ, Holstege FC, Cuppen E, de Bruin A
Nucleic acids research 2012 Apr;40(8):3511-23
Nucleic acids research 2012 Apr;40(8):3511-23
Mutant telomerase RNAs induce DNA damage and apoptosis via the TRF2-ATM pathway in telomerase-overexpressing primary fibroblasts.
Mahalingam D, Tay LL, Tan WH, Chai JH, Wang X
The FEBS journal 2011 Oct;278(19):3724-38
The FEBS journal 2011 Oct;278(19):3724-38
Loss of fibulin-5 binding to beta1 integrins inhibits tumor growth by increasing the level of ROS.
Schluterman MK, Chapman SL, Korpanty G, Ozumi K, Fukai T, Yanagisawa H, Brekken RA
Disease models & mechanisms 2010 May-Jun;3(5-6):333-42
Disease models & mechanisms 2010 May-Jun;3(5-6):333-42
Chemopreventive agent 3,3'-diindolylmethane selectively induces proteasomal degradation of class I histone deacetylases.
Li Y, Li X, Guo B
Cancer research 2010 Jan 15;70(2):646-54
Cancer research 2010 Jan 15;70(2):646-54
Potentiation of temozolomide cytotoxicity by inhibition of DNA polymerase beta is accentuated by BRCA2 mutation.
Stachelek GC, Dalal S, Donigan KA, Campisi Hegan D, Sweasy JB, Glazer PM
Cancer research 2010 Jan 1;70(1):409-17
Cancer research 2010 Jan 1;70(1):409-17
Dual phosphoinositide 3-kinase/mammalian target of rapamycin blockade is an effective radiosensitizing strategy for the treatment of non-small cell lung cancer harboring K-RAS mutations.
Konstantinidou G, Bey EA, Rabellino A, Schuster K, Maira MS, Gazdar AF, Amici A, Boothman DA, Scaglioni PP
Cancer research 2009 Oct 1;69(19):7644-52
Cancer research 2009 Oct 1;69(19):7644-52
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: gamma H2AX [p Ser139] Antibody [NB100-2280] - Simple Western lane view shows a specific band for gamma H2AX in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: gamma H2AX [p Ser139] Antibody [NB100-2280] - Electropherogram image(s) of corresponding Simple Western lane view. Gamma H2AX antibody was used at 1:20 dilution on Jurkat lysate(s).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gamma H2AX [p Ser139] Antibody [NB100-2280] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit antigamma H2AX used at a dilution of 1:250. Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gamma H2AX [p Ser139] Antibody [NB100-2280] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-gamma H2AX used at a dilution of 1:250. Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-2280] - Detection of Mouse gamma-H2AX by Immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibodies: Affinity purified rabbit anti-gamma-H2AX NB100-2280. Detection: DAB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-2280] - Telomere dysfunctional induced foci (TIF) in HBV-related multistep hepatocarcinogenesis and the correlations thereof with stathmin and elongation factor 1alpha (EF1alpha) expression. A. Representative features of colocalization of gamma H2AX and telomeric DNA in defined lesions of human multistep hepatocarcinogenesis. TIF are indicated by colored arrowheads: blue, DAPI; green, gamma H2AX; red, telomeres; yellow, TIF. Image collected and cropped by CiteAb from the following publication (http://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-12-154) licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-2280] - Biological and molecular analysis of cutaneous wound healing in p73+/+ and p73-/- mice. Representative micrographs of immunohistochemistry (IHC) staining for gamma-H2AX in skin specimens from p73+/+ and p73-/- mice 10 days after wounding. All scale bars represent 50 um. Regions of the skin are labeled as: IFE, HF, epidermal wound edge (WE), and newly-formed epidermis of the wound (W); and the dotted line indicates the border between the WE and W. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0218458), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gamma H2AX [p Ser139] Antibody [NB100-2280] - Detection of human gamma H2AX [p Ser139] Antibody by immunohistochemistry. Sample: FFPE section of human seminoma. Antibodies: Affinity purified rabbit anti-gamma-H2A used at a dilution of 1:500. Detection: DAB.
