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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunoprecipitation [1]
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- Product number
- NB100-2584 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2584, RRID:AB_10002040
- Product name
- Rabbit Polyclonal Spt6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The cellular protein SPT6 is required for efficient replication of human cytomegalovirus.
Cygnar D, Hagemeier S, Kronemann D, Bresnahan WA
Journal of virology 2012 Feb;86(4):2011-20
Journal of virology 2012 Feb;86(4):2011-20
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: Spt6 Antibody [NB100-2584] - Detection of Human SUPT6H by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUPT6H antibodies NB100-2583, NB100-2584 and another SUPT6H Ab used for IP at 3 mcg/mg lysate (A and B). Detection: Chemiluminescence with exposure times of 30 seconds (A and B). * - The asterisk marks a protein with which BL3468 interacts in IP and WB. This band is likely to be a cross-reacting protein.
