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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB11329 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11329, RRID:AB_1716729
- Product name
- Ubash3b polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Ubash3b.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Suppressors of T-cell receptor signaling Sts-1 and Sts-2 bind to Cbl and inhibit endocytosis of receptor tyrosine kinases.
Regulation of ZAP-70 activation and TCR signaling by two related proteins, Sts-1 and Sts-2.
Identification, cDNA cloning, and targeted deletion of p70, a novel, ubiquitously expressed SH3 domain-containing protein.
Kowanetz K, Crosetto N, Haglund K, Schmidt MH, Heldin CH, Dikic I
The Journal of biological chemistry 2004 Jul 30;279(31):32786-95
The Journal of biological chemistry 2004 Jul 30;279(31):32786-95
Regulation of ZAP-70 activation and TCR signaling by two related proteins, Sts-1 and Sts-2.
Carpino N, Turner S, Mekala D, Takahashi Y, Zang H, Geiger TL, Doherty P, Ihle JN
Immunity 2004 Jan;20(1):37-46
Immunity 2004 Jan;20(1):37-46
Identification, cDNA cloning, and targeted deletion of p70, a novel, ubiquitously expressed SH3 domain-containing protein.
Carpino N, Kobayashi R, Zang H, Takahashi Y, Jou ST, Feng J, Nakajima H, Ihle JN
Molecular and cellular biology 2002 Nov;22(21):7491-500
Molecular and cellular biology 2002 Nov;22(21):7491-500
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using Ubash3b polyclonal antibody (Cat # PAB11329) shows detection of a band ~70 kDa correspondingto mouse Ubash3b.Approximately 1.0 ug of recombinant (truncated) Ubash3b-GST was separated by SDS-PAGE and probed with the primary antibody diluted to 0.3 ug/mL .
