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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-34667 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- gamma Actin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, NIH-3T3, JC, BCL-1, PC-12, Rat2.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of gamma Actin was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a gamma Actin Polyclonal Antibody (Product # PA5-34667) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using gamma Actin Polyclonal Antibody (Product # PA5-34667). Sample (30 µg of whole cell lysate). Lane A: A431. 10% SDS PAGE. gamma Actin Polyclonal Antibody (Product # PA5-34667) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using gamma Actin Polyclonal Antibody (Product # PA5-34667). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. Lane C: BCL-1. 10% SDS PAGE. gamma Actin Polyclonal Antibody (Product # PA5-34667) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human Spleen, using gamma Actin (Product # PA5-34667) antibody (10 µg/mL). Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
