NBP1-31363

antibody from Novus Biologicals

Targeting: NPAS2 bHLHe9, MOP4, PASD4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-31363

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-31363, RRID:AB_2235999

Product name

Rabbit Polyclonal NPAS2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Cyp3a11 metabolism-based chronotoxicity of brucine in mice.

Zhou Z, Lin Y, Gao L, Yang Z, Wang S, Wu B
Toxicology letters 2019 Oct 1;313:188-195

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NPAS2 Antibody [NBP1-31363] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE; antibody diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NPAS2 Antibody [NBP1-31363] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NPAS2 Antibody [NBP1-31363] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] diluted at 1:1000.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NPAS2 Antibody [NBP1-31363] - H441 xenograft, using NPAS2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: NPAS2 Antibody [NBP1-31363] - Frozen sectioned E13.5 Rat brain. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: NPAS2 Antibody [NBP1-31363] - Frozen sectioned adult mouse retina. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody 11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NPAS2 Antibody [NBP1-31363] - Mouse eye. Green: NPAS2 stained by NPAS2 antibody [C1C3] diluted at 1:250. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.