TA505187

antibody from OriGene

Targeting: UBE2S E2-EPF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505187

Provider

OriGene

Proper citation

OriGene Cat#TA505187, RRID:AB_2622906

Product name

UBE2S mouse monoclonal antibody, clone OTI4H3 (formerly 4H3)

Antibody type

Monoclonal

Description

UBE2S mouse monoclonal antibody, clone OTI4H3 (formerly 4H3)

Host

Mouse

Conjugate

Unconjugated

Epitope

UBE2S

Isotype

IgG

Antibody clone number

OTI4H3

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-UBE2S monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (RC224851, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-UBE2S mouse monoclonal antibody (TA505187) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2S(RC224851).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC224851 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UBE2S antibody(TA505187), and then analyzed by flow cytometry.

Validation comment

FC