MAB7885

antibody from Abnova Corporation

Targeting: UBE2S E2-EPF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB7885

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB7885, RRID:AB_10676018

Product name

UBE2S monoclonal antibody, clone AT2C12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against full length recombinant UBE2S.

Isotype

IgG

Antibody clone number

AT2C12

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Building ubiquitin chains: E2 enzymes at work.

Ye Y, Rape M
Nature reviews. Molecular cell biology 2009 Nov;10(11):755-64

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Two different classes of E2 ubiquitin-conjugating enzymes are required for the mono-ubiquitination of proteins and elongation by polyubiquitin chains with a specific topology.

Windheim M, Peggie M, Cohen P
The Biochemical journal 2008 Feb 1;409(3):723-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Cell lysates of 293T (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with UBE2S monoclonal antibody, clone AT2C12 (Cat # MAB7885) (1 : 250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and UBE2S monoclonal antibody, clone AT2C12 (Cat # MAB7885) (1 : 500) with Alexa 488 (Green).

Validation comment

Immunofluorescence