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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB398-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Leptin/OB Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Captures human Leptin/OB in sandwich ELISAs. In this format, this antibody shows less than 0.1% cross-reactivity with recombinant mouse (rm) Leptin/OB and rrLeptin/OB.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P41159- Isotype
- IgG
- Antibody clone number
- 44802
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Regulatory T cell proliferative potential is impaired in human autoimmune disease.
Digital microfluidic assay for protein detection.
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
A multiplex immunoassay for human adipokine profiling.
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects.
Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis.
Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
Carbone F, De Rosa V, Carrieri PB, Montella S, Bruzzese D, Porcellini A, Procaccini C, La Cava A, Matarese G
Nature medicine 2014 Jan;20(1):69-74
Nature medicine 2014 Jan;20(1):69-74
Digital microfluidic assay for protein detection.
Mok J, Mindrinos MN, Davis RW, Javanmard M
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 11;111(6):2110-5
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 11;111(6):2110-5
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
A multiplex immunoassay for human adipokine profiling.
Schipper HS, de Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clinical chemistry 2010 Aug;56(8):1320-8
Clinical chemistry 2010 Aug;56(8):1320-8
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation 2009 Jun 27;87(12):1814-20
Transplantation 2009 Jun 27;87(12):1814-20
Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects.
Madsen EL, Bruun JM, Skogstrand K, Hougaard DM, Christiansen T, Richelsen B
Metabolism: clinical and experimental 2009 Jul;58(7):946-53
Metabolism: clinical and experimental 2009 Jul;58(7):946-53
Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
Emsley HC, Smith CJ, Gavin CM, Georgiou RF, Vail A, Barberan EM, Illingworth K, Scarth S, Wickramasinghe V, Hoadley ME, Rothwell NJ, Tyrrell PJ, Hopkins SJ
BMC neurology 2007 Feb 28;7:5
BMC neurology 2007 Feb 28;7:5
Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis.
Wunder DM, Mueller MD, Birkhäuser MH, Bersinger NA
Journal of assisted reproduction and genetics 2005 Jun;22(6):257-64
Journal of assisted reproduction and genetics 2005 Jun;22(6):257-64
Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
European cytokine network 2002 Apr-Jun;13(2):225-9
European cytokine network 2002 Apr-Jun;13(2):225-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by Leptin/OB and Neutralization by Human Leptin/OB Antibody. Recombinant Human Leptin/OB (Catalog # 398-LP) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with human Leptin R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Leptin/OB (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Human Leptin/OB Monoclonal Antibody (Catalog # MAB398). The ND50 is typically 0.4-1.6 µg/mL.
