844803

antibody from BioLegend

Targeting: CTNNA1 CAP102

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

844803

Provider

BioLegend

Proper citation

BioLegend Cat#844803, RRID:AB_2565809

Product name

Ultra-LEAF™ Purified anti-α-E-Catenin

Antibody type

Monoclonal

Antigen

Recombinant human MBP-alpha catenin fusion protein.

Description

α-catenin associates with the cytoplasmic domain of a variety of plasma membrane spanning cadherins that mediate cell-cell adhesion. The association of catenins to cadherins produces a complex that is linked to the actin filament network, which seems to be of primary importance for their cell-adhesion properties. Catenin can associate with both E- and N-cadherins. It was originally thought to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and α-catenin was shown not to bind to F-actin when assembled in the complex suggesting a more complex, dynamic linkage between actin and these adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

1G5

Vial size

100 µg

Concentration

1.0 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

MeOH fixed HeLa cells were stained with α-Catenin (clone 1G5) at 1 µg/ml. Goat-anti mouse Alexa Fluor® 594 secondary was used and the cells were counterstained using DAPI.

Conjugate

Unconjugated