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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-233-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human FGF basic/FGF2/bFGF Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human FGF basic/FGF2/bFGF in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human FGF acidic is observed. Neutralizes the biological activity of recombinant human FGF basic/FGF2/bFGF. It will also neutralize the biological effects of bovine FGF basic/FGF2/bFGF, but it has no effect on recombinant human or bovine FGF acidic.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Thyroxine (T4) may promote re-epithelialisation and angiogenesis in wounded human skin ex vivo.
Reciprocal cellular cross-talk within the tumor microenvironment promotes oncolytic virus activity.
Tricyclic antidepressant amitriptyline indirectly increases the proliferation of adult dentate gyrus-derived neural precursors: an involvement of astrocytes.
Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity.
Dedicated epithelial recipient cells determine pigmentation patterns.
Zhang GY, Langan EA, Meier NT, Funk W, Siemers F, Paus R
PloS one 2019;14(3):e0212659
PloS one 2019;14(3):e0212659
Reciprocal cellular cross-talk within the tumor microenvironment promotes oncolytic virus activity.
Ilkow CS, Marguerie M, Batenchuk C, Mayer J, Ben Neriah D, Cousineau S, Falls T, Jennings VA, Boileau M, Bellamy D, Bastin D, de Souza CT, Alkayyal A, Zhang J, Le Boeuf F, Arulanandam R, Stubbert L, Sampath P, Thorne SH, Paramanthan P, Chatterjee A, Strieter RM, Burdick M, Addison CL, Stojdl DF, Atkins HL, Auer RC, Diallo JS, Lichty BD, Bell JC
Nature medicine 2015 May;21(5):530-6
Nature medicine 2015 May;21(5):530-6
Tricyclic antidepressant amitriptyline indirectly increases the proliferation of adult dentate gyrus-derived neural precursors: an involvement of astrocytes.
Boku S, Hisaoka-Nakashima K, Nakagawa S, Kato A, Kajitani N, Inoue T, Kusumi I, Takebayashi M
PloS one 2013;8(11):e79371
PloS one 2013;8(11):e79371
Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity.
Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ
Brain : a journal of neurology 2010 Aug;133(Pt 8):2232-47
Brain : a journal of neurology 2010 Aug;133(Pt 8):2232-47
Dedicated epithelial recipient cells determine pigmentation patterns.
Weiner L, Han R, Scicchitano BM, Li J, Hasegawa K, Grossi M, Lee D, Brissette JL
Cell 2007 Sep 7;130(5):932-42
Cell 2007 Sep 7;130(5):932-42
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by FGF basic/FGF2 and Neutralization by Human FGF basic/FGF2 Antibody. Recombinant Human FGF basic/FGF2 146 aa (Catalog # 233-FB) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF basic/FGF2 146 aa (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF basic/FGF2 Polyclonal Antibody (Catalog # AB-233-NA). The ND50 is typically 0.5-1.0 µg/mL.
