BAF233

antibody from R&D Systems

Targeting: FGF2 FGFB

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BAF233

Provider

R&D Systems

Product name

Human FGF basic/FGF2/bFGF Biotinylated Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human FGF basic/FGF2/bFGF in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) FGF acidic and rhFGF-7 is observed under non-reducing conditions.

Reactivity

Human

Host

Goat

Conjugate

Biotin

Antigen sequence

P09038


Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

FGF basic/FGF2 in Human Ovarian Cancer Tissue. FGF basic/FGF2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human FGF basic/FGF2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF233) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

FGF basic/FGF2 in Human Ovary. FGF basic/FGF2 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Human FGF basic/FGF2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF233) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.