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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MAB10601 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10601, RRID:AB_11189390
- Product name
- KRT8 monoclonal antibody, clone 8A5D12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant KRT8.
- Isotype
- IgG
- Antibody clone number
- 8A5D12
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) against A-549 (1) , HeLa (2) , MCF-7 (3) , A-431 (4) and HepG2 (5) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (upper) and HepG2 cells (bottom) using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) (green) , showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) , lung cancer (B) and ovarian cancer tissue (C) , showing membrane and cytoplasmic localization with DAB staining using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney tissues using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
