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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- TA300410 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA300410, RRID:AB_2281149
- Product name
- Rabbit Monoclonal Antibody against KRT8 (Clone E431-2)
- Antibody type
- Monoclonal
- Description
- Rabbit Monoclonal Antibody against KRT8 (Clone E431-2)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- KRT8
- Isotype
- IgG
- Antibody clone number
- E431-2
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 (phospho S73) antibody [E431-2]; Immunofluorescent staining of A431 cells using anti-Phospho-Keratin 8 diluted 1/250.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 (phospho S73) antibody [E431-2]; Ab32579 staining cells from a human pancreatic cell line by ICC/IF. The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. Ab32579 was diluted 1/25 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor#174; 488 conjugated donkey anti-rabbit antibody was used as the secondary.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 8 (phospho S73) antibody [E431-2]; IHC-P analysis (with antigen retrieval) of liver carcinoma using anti-Phospho-Keratin 8 diluted 1/500.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IHC of Mouse lung adenoma, staining Cytokeratin 8 (phospho S73) with TA300410.Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in PBS) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.
- Validation comment
- IHC
