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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX84497 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84497, RRID:AB_10729438
- Product name
- Fumarate hydratase antibody [1F10]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat, Canine
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FH monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-FH mouse monoclonal antibody (GTX84497) immunofluorescent staining of COS7 cells transiently transfected with FH
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded colon tissue using anti-FHmouse monoclonal antibody. (GTX84497, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FHmouse monoclonal antibody. (GTX84497, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-FHmouse monoclonal antibody. (GTX84497, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either Fumarate hydratase overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FH antibody (GTX84497), and then analyzed by flow cytometry.
