213851

antibody from Invitrogen Antibodies

Targeting: IFNAR2 IFNABR

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

213851

Provider

Invitrogen Antibodies

Product name

IFNAR2 Monoclonal Antibody (MMHAR-2)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody neutralizes human IFN-alpha receptor, interacting with the extracellular domain. It binds to human IFN-alpha receptor with high affinity and blocks biological action of Type I IFNs. To measure bioactivity, interferon was titrated with the use of a cytopathic effect inhibition assay, where one neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha to a 50% endpoint. In this antiviral assay, approximately one unit/mL of interferon was necessary to produce a cytopathic effect of 50%.

Antibody clone number

MMHAR-2

Concentration

0.5 mg/mL

References

Submitted references

Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages.

Dupont M, Souriant S, Balboa L, Vu Manh TP, Pingris K, Rousset S, Cougoule C, Rombouts Y, Poincloux R, Ben Neji M, Allers C, Kaushal D, Kuroda MJ, Benet S, Martinez-Picado J, Izquierdo-Useros N, Sasiain MDC, Maridonneau-Parini I, Neyrolles O, Vérollet C, Lugo-Villarino G
eLife 2020 Mar 30;9

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Human monocyte-derived macrophages inhibit HCMV spread independent of classical antiviral cytokines.

Becker J, Kinast V, Döring M, Lipps C, Duran V, Spanier J, Tegtmeyer PK, Wirth D, Cicin-Sain L, Alcamí A, Kalinke U
Virulence 2018;9(1):1669-1684

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PARP1 depletion induces RIG-I-dependent signaling in human cancer cells.

Ghosh R, Roy S, Franco S
PloS one 2018;13(3):e0194611

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Priming and Activation of Inflammasome by Canarypox Virus Vector ALVAC via the cGAS/IFI16-STING-Type I IFN Pathway and AIM2 Sensor.

Liu F, Niu Q, Fan X, Liu C, Zhang J, Wei Z, Hou W, Kanneganti TD, Robb ML, Kim JH, Michael NL, Sun J, Soong L, Hu H
Journal of immunology (Baltimore, Md. : 1950) 2017 Nov 1;199(9):3293-3305

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Targeting type I interferon-mediated activation restores immune function in chronic HIV infection.

Zhen A, Rezek V, Youn C, Lam B, Chang N, Rick J, Carrillo M, Martin H, Kasparian S, Syed P, Rice N, Brooks DG, Kitchen SG
The Journal of clinical investigation 2017 Jan 3;127(1):260-268

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Endothelial IL-33 Expression Is Augmented by Adenoviral Activation of the DNA Damage Machinery.

Stav-Noraas TE, Edelmann RJ, Poulsen LC, Sundnes O, Phung D, Küchler AM, Müller F, Kamen AA, Haraldsen G, Kaarbø M, Hol J
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 15;198(8):3318-3325

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Replication of Human Norovirus RNA in Mammalian Cells Reveals Lack of Interferon Response.

Qu L, Murakami K, Broughman JR, Lay MK, Guix S, Tenge VR, Atmar RL, Estes MK
Journal of virology 2016 Oct 1;90(19):8906-23

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Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway.

Bowers NL, Helton ES, Huijbregts RP, Goepfert PA, Heath SL, Hel Z
PLoS pathogens 2014 Mar;10(3):e1003993

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

THP-1 cells were infected with virus in presence of IFNAR2 antibody (Product # 213851, final concentration: 10 µg/mL). Cells were collected at 24 hours post-infection followed by RNA extraction and RT-qPCR. The mRNA level of AIM2, an interferon-inducible protein, was measured by real-time PCR. Data courtesy of Antibody Data Exchange Program.