NBP2-24867

antibody from Novus Biologicals

Targeting: CD3E

Flow cytometry (Supportive data in Antibodypedia)

Functional assay (Data presented on provider website)

Antibody Data

Product number

NBP2-24867

Provider

Novus Biologicals

Product name

Mouse Monoclonal CD3 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

PD-1/CTLA-4 Blockade Inhibits Epstein-Barr Virus-Induced Lymphoma Growth in a Cord Blood Humanized-Mouse Model.

Ma SD, Xu X, Jones R, Delecluse HJ, Zumwalde NA, Sharma A, Gumperz JE, Kenney SC
PLoS pathogens 2016 May;12(5):e1005642

---

Knockout of Epstein-Barr virus BPLF1 retards B-cell transformation and lymphoma formation in humanized mice.

Whitehurst CB, Li G, Montgomery SA, Montgomery ND, Su L, Pagano JS
mBio 2015 Oct 20;6(5):e01574-15

---

Cocaine-mediated impact on HIV infection in humanized BLT mice.

Kim SG, Lowe EL, Dixit D, Youn CS, Kim IJ, Jung JB, Rovner R, Zack JA, Vatakis DN
Scientific reports 2015 Jun 18;5:10010

---

LMP1-deficient Epstein-Barr virus mutant requires T cells for lymphomagenesis.

Ma SD, Xu X, Plowshay J, Ranheim EA, Burlingham WJ, Jensen JL, Asimakopoulos F, Tang W, Gulley ML, Cesarman E, Gumperz JE, Kenney SC
The Journal of clinical investigation 2015 Jan;125(1):304-15

---

TAp73 opposes tumor angiogenesis by promoting hypoxia-inducible factor 1α degradation.

Amelio I, Inoue S, Markert EK, Levine AJ, Knight RA, Mak TW, Melino G
Proceedings of the National Academy of Sciences of the United States of America 2015 Jan 6;112(1):226-31

---

Mitochondria-derived hydrogen peroxide selectively enhances T cell receptor-initiated signal transduction.

Gill T, Levine AD
The Journal of biological chemistry 2013 Sep 6;288(36):26246-55

---

An Epstein-Barr Virus (EBV) mutant with enhanced BZLF1 expression causes lymphomas with abortive lytic EBV infection in a humanized mouse model.

Ma SD, Yu X, Mertz JE, Gumperz JE, Reinheim E, Zhou Y, Tang W, Burlingham WJ, Gulley ML, Kenney SC
Journal of virology 2012 Aug;86(15):7976-87

---

Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.

Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9

---

Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.

Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Cell Surface): CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-24867. Detection of CD3 expression in hPBMc's gated to lymphocyte population at 1 x 10^6 cells/ml. Cells were stained with NBP2-24867AF488 (orange) conjugate at a 1:500 dilution. Results are shown with mIgG2 (AF488 conjugate) isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Cell Surface): CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Analysis using the PE conjugate of NBP2-24867. Detection of CD3 expression in hPBMC's gated to lymphocyte population at 1 x 10^6 cells/ml. Cells were stained with NBP2-24867PE (orange) conjugate at a 1:500 dilution. Results are shown with mIgG (PE conjuga

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-24867. Staining of human PBMC. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Analysis of CD3+ hPMBCs using the APC conjugate of this antibody. A cell surface stain was performed on CD3+ hPBMCs with V alpha 24 J alpha 18 TCR antibody (6B11) NBP2-00267PE (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. A co-stain was also performed using CD3 antibody NBP2-24867APC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Analysis using Alexa Fluor (R) 488 conjugate of NBP2-24867. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Surface staining of human PBMC with CD3 antibody (red) grown in serum-free media and isotype control (green) at 0.25 ug/10^6 cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Surface staining of human PBMC with CD3 antibody (red) and isotype control (green) at 0.2 ug/10^6 cells.