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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 368302 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#368302, RRID:AB_2566176
- Product name
- Anti-human CD3 FITC/CD4 PE/CD45 PerCP Cocktail
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- CD3: SK7 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD4: SK3 antibody reacts with CD4, a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD45: HI30 antibody reacts with CD45, a 180-240 kD single chain type I membrane glycoprotein which is also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets.
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- SK7/SK3/HI30
- Vial size
- 50 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes were stained with anti-human CD3 FITC/CD4 PE/CD45 PerCP cocktail. The top dot plot shows SSC Vs CD45+ profile. The events in the bottom CD3 Vs CD4 dot plot are gated on lymphocytes as shown in the top figure.
