Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- MAB4841-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 17A2
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Crucial role of OX40/OX40L signaling in a murine model of asthma.
Nitrosative damage during retrovirus infection-induced neuropathic pain.
A Novel C-Terminal Mutation in Gsdma3 (C+/H-) Leads to Alopecia and Corneal Inflammatory Response in Mice.
CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration.
Aloperine Protects Mice against DSS-Induced Colitis by PP2A-Mediated PI3K/Akt/mTOR Signaling Suppression.
Characterisation of Immune and Neuroinflammatory Changes Associated with Chemotherapy-Induced Peripheral Neuropathy.
Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2.
The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells.
Temporal Expression of Bim Limits the Development of Agonist-Selected Thymocytes and Skews Their TCRβ Repertoire.
Metronomic Doses of Temozolomide Enhance the Efficacy of Carbon Nanotube CpG Immunotherapy in an Invasive Glioma Model.
Selective suppression of the α isoform of p38 MAPK rescues late-stage tau pathology.
Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif.
LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis.
TGF-β promotes immune responses in the presence of mesenchymal stem cells.
Ninjurin1 deficiency attenuates susceptibility of experimental autoimmune encephalomyelitis in mice.
Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac.
Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
Identification of a murine CD28 dileucine motif that suppresses single-chain chimeric T-cell receptor expression and function.
Lei W, Zeng D, Liu G, Zhu Y, Wang J, Wu H, Jiang J, Huang J
Molecular medicine reports 2018 Mar;17(3):4213-4220
Molecular medicine reports 2018 Mar;17(3):4213-4220
Nitrosative damage during retrovirus infection-induced neuropathic pain.
Chauhan P, Sheng WS, Hu S, Prasad S, Lokensgard JR
Journal of neuroinflammation 2018 Mar 5;15(1):66
Journal of neuroinflammation 2018 Mar 5;15(1):66
A Novel C-Terminal Mutation in Gsdma3 (C+/H-) Leads to Alopecia and Corneal Inflammatory Response in Mice.
Swirski S, Röger C, Pienkowska-Schelling A, Ihlenburg C, Fischer G, May O, Vorm M, Owczarek-Lipska M, Neidhardt J
Investigative ophthalmology & visual science 2018 Jan 1;59(1):561-571
Investigative ophthalmology & visual science 2018 Jan 1;59(1):561-571
CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration.
Deyhle MR, Hafen PS, Parmley J, Preece CN, Robison M, Sorensen JR, Jackson B, Eggett DL, Hancock CR, Hyldahl RD
Physiological reports 2018 Apr;6(8):e13689
Physiological reports 2018 Apr;6(8):e13689
Aloperine Protects Mice against DSS-Induced Colitis by PP2A-Mediated PI3K/Akt/mTOR Signaling Suppression.
Fu X, Sun F, Wang F, Zhang J, Zheng B, Zhong J, Yue T, Zheng X, Xu JF, Wang CY
Mediators of inflammation 2017;2017:5706152
Mediators of inflammation 2017;2017:5706152
Characterisation of Immune and Neuroinflammatory Changes Associated with Chemotherapy-Induced Peripheral Neuropathy.
Makker PG, Duffy SS, Lees JG, Perera CJ, Tonkin RS, Butovsky O, Park SB, Goldstein D, Moalem-Taylor G
PloS one 2017;12(1):e0170814
PloS one 2017;12(1):e0170814
Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2.
Fischer R, Marsal J, Guttà C, Eisler SA, Peters N, Bethea JR, Pfizenmaier K, Kontermann RE
Scientific reports 2017 Jul 26;7(1):6607
Scientific reports 2017 Jul 26;7(1):6607
The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells.
Pan W, Hao WT, Shen YJ, Li XY, Wang YJ, Sun FF, Yin JH, Zhang J, Tang RX, Cao JP, Zheng KY
Parasites & vectors 2017 Jul 21;10(1):348
Parasites & vectors 2017 Jul 21;10(1):348
Temporal Expression of Bim Limits the Development of Agonist-Selected Thymocytes and Skews Their TCRβ Repertoire.
Li KP, Fähnrich A, Roy E, Cuda CM, Grimes HL, Perlman HR, Kalies K, Hildeman DA
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 1;198(1):257-269
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 1;198(1):257-269
Metronomic Doses of Temozolomide Enhance the Efficacy of Carbon Nanotube CpG Immunotherapy in an Invasive Glioma Model.
Ouyang M, White EE, Ren H, Guo Q, Zhang I, Gao H, Yanyan S, Chen X, Weng Y, Da Fonseca A, Shah S, Manuel ER, Zhang L, Vonderfecht SL, Alizadeh D, Berlin JM, Badie B
PloS one 2016;11(2):e0148139
PloS one 2016;11(2):e0148139
Selective suppression of the α isoform of p38 MAPK rescues late-stage tau pathology.
Maphis N, Jiang S, Xu G, Kokiko-Cochran ON, Roy SM, Van Eldik LJ, Watterson DM, Lamb BT, Bhaskar K
Alzheimer's research & therapy 2016 Dec 15;8(1):54
Alzheimer's research & therapy 2016 Dec 15;8(1):54
Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif.
Fan M, Zhang Y, Huang Z, Liu J, Guo X, Zhang H, Luo H
PloS one 2014;9(9):e108253
PloS one 2014;9(9):e108253
LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis.
Nakamura T, Hamuro J, Takaishi M, Simmons S, Maruyama K, Zaffalon A, Bentley AJ, Kawasaki S, Nagata-Takaoka M, Fullwood NJ, Itami S, Sano S, Ishii M, Barrandon Y, Kinoshita S
The Journal of clinical investigation 2014 Jan;124(1):385-97
The Journal of clinical investigation 2014 Jan;124(1):385-97
TGF-β promotes immune responses in the presence of mesenchymal stem cells.
Xu C, Yu P, Han X, Du L, Gan J, Wang Y, Shi Y
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 1;192(1):103-9
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 1;192(1):103-9
Ninjurin1 deficiency attenuates susceptibility of experimental autoimmune encephalomyelitis in mice.
Ahn BJ, Le H, Shin MW, Bae SJ, Lee EJ, Wee HJ, Cha JH, Lee HJ, Lee HS, Kim JH, Kim CY, Seo JH, Lo EH, Jeon S, Lee MN, Oh GT, Yin GN, Ryu JK, Suh JK, Kim KW
The Journal of biological chemistry 2014 Feb 7;289(6):3328-38
The Journal of biological chemistry 2014 Feb 7;289(6):3328-38
Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac.
Vang AG, Housley W, Dong H, Basole C, Ben-Sasson SZ, Kream BE, Epstein PM, Clark RB, Brocke S
The Biochemical journal 2013 Dec 15;456(3):463-73
The Biochemical journal 2013 Dec 15;456(3):463-73
Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
De Palma AM, Verbeken E, Van Aelst I, Van den Steen PE, Opdenakker G, Neyts J
Virology 2008 Dec 5;382(1):20-7
Virology 2008 Dec 5;382(1):20-7
Identification of a murine CD28 dileucine motif that suppresses single-chain chimeric T-cell receptor expression and function.
Nguyen P, Moisini I, Geiger TL
Blood 2003 Dec 15;102(13):4320-5
Blood 2003 Dec 15;102(13):4320-5
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD3 in Mouse Splenocytes. CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD3 in Mouse Splenocytes. CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD3 in Mouse Brain. CD3 was detected in perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) using 10 µg/mL Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.