LS-C355241

antibody from LSBio

Targeting: CEACAM5 CD66e, CEA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

LS-C355241

Provider

LSBio

Product name

CEACAM5 / CD66e Antibody (clone 1105) LS-C355241

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1105

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of Carcinoembryonic Antigen (CEA) was performed by loading 20ug of BxPC-3, HeLa, and HepG2 whole cell lysates per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. CEA (with varying degrees of glycosylation) was detected at ~77-180kD using a CEA monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.