ABIN108727

antibody from antibodies-online

Targeting: CEACAM5 CD66e, CEA

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN108727

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN108727, RRID:AB_10952637

Product name

anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) antibody

Antibody type

Monoclonal

Antigen

immunisation with extracted protein of CEACAM5

Description

Protein G

Reactivity

Human

Isotype

IgG

Antibody clone number

26-3-13

Vial size

100 μg

Concentration

1 mg/mL

Storage

short term: 2°C - 8°C, long term: -20°C

Handling

Avoid repeated freezing and thawing.

References

Submitted references

The CEA/CD3-bispecific antibody MEDI-565 (MT111) binds a nonlinear epitope in the full-length but not a short splice variant of CEA.

Peng L, Oberst MD, Huang J, Brohawn P, Morehouse C, Lekstrom K, Baeuerle PA, Wu H, Yao Y, Coats SR, Dall'Acqua W, Damschroder M, Hammond SA
PloS one 2012;7(5):e36412

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Comparison of surgical and endoscopic sample collection for pancreatic cyst fluid biomarker identification.

Partyka K, McDonald M, Maupin KA, Brand R, Kwon R, Simeone DM, Allen P, Haab BB
Journal of proteome research 2012 May 4;11(5):2904-11

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Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.

Drukker M, Tang C, Ardehali R, Rinkevich Y, Seita J, Lee AS, Mosley AR, Weissman IL, Soen Y
Nature biotechnology 2012 May 27;30(6):531-42

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Differential glycosylation of MUC1 and CEACAM5 between normal mucosa and tumour tissue of colon cancer patients.

Saeland E, Belo AI, Mongera S, van Die I, Meijer GA, van Kooyk Y
International journal of cancer. Journal international du cancer 2012 Jul 1;131(1):117-28

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Characterization of gastric adenocarcinoma cell lines established from CEA424/SV40 T antigen-transgenic mice with or without a human CEA transgene.

Nöckel J, van den Engel NK, Winter H, Hatz RA, Zimmermann W, Kammerer R
BMC cancer 2006 Mar 14;6:57

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The tumour suppressor gene CEACAM1 is completely but reversibly downregulated in renal cell carcinoma.

Kammerer R, Riesenberg R, Weiler C, Lohrmann J, Schleypen J, Zimmermann W
The Journal of pathology 2004 Nov;204(3):258-67

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Granulocyte CEACAM3 is a phagocytic receptor of the innate immune system that mediates recognition and elimination of human-specific pathogens.

Schmitter T, Agerer F, Peterson L, Munzner P, Hauck CR
The Journal of experimental medicine 2004 Jan 5;199(1):35-46

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The tumorigenic potential of human CX-1 colon adenocarcinoma cells depends on carcinoembryonic antigen (CEACAM5) expression.

Baczyńska D, Wietrzyk J, Madej J, Krop-Watorek A, Dabrowska A, Widerak K, Opolski A, Ugorski M
Cellular & molecular biology letters 2003;8(2):471-86

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Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas.

Schölzel S, Zimmermann W, Schwarzkopf G, Grunert F, Rogaczewski B, Thompson J
The American journal of pathology 2000 Feb;156(2):595-605

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CEA-related proteins on human urothelial cell lines of different transformation grades.

Krop-Watorek A, Laskowska A, Salwa J, Kłopocki AG, Grunert F, Ugorski M
Cancer letters 1999 May 3;139(1):15-22

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A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members.

Thompson J, Mössinger S, Reichardt V, Engels U, Beauchemin N, Kommoss F, von Kleist S, Zimmermann W
International journal of cancer. Journal international du cancer 1993 Sep 9;55(2):311-9

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Effects of human bone marrow stroma on the growth of human tumor cells.

Strobel ES, Strobel HG, Bross KJ, Winterhalter B, Fiebig HH, Schildge JU, Löhr GW
Cancer research 1989 Feb 15;49(4):1001-7

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Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns.

Grunert F, AbuHarfeil N, Schwarz K, von Kleist S
International journal of cancer. Journal international du cancer 1985 Sep 15;36(3):357-62

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

FACS analysis of BOSC23 cells using 26/3/13. BOSC23 cells were transiently transfected with an expres-sion vector encoding either CEACAM5 (red curve) or an irrelevant protein (control transfectant). Binding of 26/3/13 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM5 transfected cells.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Specificity testing of 26/3/13. BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM5-8. Recognition of CEACAM3, 4 and of a recombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa (CEACAM3, PSG) and CHO cells (CEACAM4), respectively. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: TET2; PSG: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified 26/3/13 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM5 expressing cells (red curves).