DM1215

antibody from Acris Antibodies GmbH

Targeting: CEACAM5 CD66e, CEA

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

DM1215

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#DM1215, RRID:AB_977066

Product name

anti CD66e / CEACAM5

Antibody type

Monoclonal

Antigen

Immunisation with extracted protein of CEACAM5

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

26/5/1

Vial size

0.1 mg

Concentration

1.0 mg/ml

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Fig.1: FACS analysis of BOSC23 cells using 26/5/1 DM1215. BOSC23 cells were transiently transfected with an expres-sion vector encoding either CEACAM5 (red curve) or an irrelevantprotein (control transfectant). Binding of 26/5/1 was detected witha PE conjugated secondary antibody. A positive signal wasobtained only with CEACAM5 transfected cells.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Fig2: Members of the CEA family were expressed on BOSC cells after transient transfectionwith expression vectors containing either the cDNA of CEACAM1, 5, 6 or 8. Recognition ofCEACAM3 and of a recombinant transmembrane-anchored PSG1 fusion protein was testedon stably transfected HeLa cells. Expression of the constructs was confirmed with monoclonalantibodies known to recognize the corresponding proteins (CEACAM1, 3, 5 and 6: D14HD11;CEACAM8: 80H3; PSG: BAP1; green curves). An irrelevant monoclonal antibody served as anegative control (black curves). For specificity testing, protein G purified 26/5/1 was tested onall CEACAM transfectants. A positive signal was only obtained with CEACAM5 expressingcells (red curves).