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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- EB07478 - Provider product page
- Provider
- Everest Biotech
- Proper citation
- Everest Biotech Cat#EB07478, RRID:AB_2109788
- Product name
- Goat anti-GFAP Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-GFAP Antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Antigen sequence
C-DGEVIKESKQEHKD- Epitope
- C Terminus
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB07478 (0.3µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB07478 (5ug/ml) as the reporter with EB002015 as the capture rabbit antibody (5ug/ml). Please note this data was obtained using a previous batch.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB07478 (2µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
