Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- LS-C149947 - Provider product page
- Provider
- LSBio
- Product name
- Anti-FN1 / Fibronectin Antibody LS-C149947
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 25µg
- Storage
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Fibronectin in A549 Human Cell Line. Fibronectin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/ml TGF-beta for 48 hours using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue).