PA1-27507

antibody from Invitrogen Antibodies

Targeting: FN1 CIG, FINC, GFND2, LETS, MSF

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-27507

Provider

Invitrogen Antibodies

Product name

Fibronectin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Reactivity

Human, Mouse, Rat

Host

Chicken/Avian

Isotype

IgY

Vial size

50 µg

Concentration

1.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Lung cancer cell-derived EDA-containing fibronectin induces an inflammatory response from monocytes and promotes metastatic tumor microenvironment.

Amin A, Mokhdomi TA, Bukhari S, Wani Z, Chikan NA, Shah BA, Koul AM, Majeed U, Farooq F, Qadri A, Qadri RA
Journal of cellular biochemistry 2021 May;122(5):562-576

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

2 Figure Fibronectin specifically secreted by A549 lung carcinoma cells contains EDA. (A) Conditioned medium was harvested after 24 h and evaluated for expression of fibronectin by immunoblotting using commercial polyclonal antifibronectin antibody. Ponceau staining of blot indicates equal loading. (B) Semiquantitative reverse transcription PCR showing differential expression of EDA and EDB exons in A549, HT-29, and THP-1 cell lines. Expression of tubulin was used to indicate equal loading. (C) Immunoblot showing expression of EDA in fibronectin secreted by A549 cells. (D) Immunoblot showing expression of EDA and fibronectin in conditioned medium of EDA knocked down A549 cells. Ponceau staining of blot indicates equal loading. PCR, polymerase chain reaction