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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC1918P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Fibronectin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Fibronectin in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions.
Purushothaman A, Bandari SK, Liu J, Mobley JA, Brown EE, Sanderson RD
The Journal of biological chemistry 2016 Jan 22;291(4):1652-63
The Journal of biological chemistry 2016 Jan 22;291(4):1652-63
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Fibronectin in WS-1 Human Cell Line by Flow Cytometry. WS-1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin PE-conjugated Monoclonal Antibody (Catalog # IC1918P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
