NBP1-91258

antibody from Novus Biologicals

Targeting: FN1 CIG, FINC, GFND2, LETS, MSF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-91258

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-91258, RRID:AB_11039761

Product name

Rabbit Polyclonal Fibronectin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Feline, Porcine

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Emergency myelopoiesis contributes to immune cell exhaustion and pulmonary vascular remodelling.

Fu C, Lu Y, Williams MA, Brantly ML, Ventetuolo CE, Morel LM, Mehrad B, Scott EW, Bryant AJ
British journal of pharmacology 2021 Jan;178(1):187-202

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circACTR2: A Novel Mechanism Regulating High Glucose-Induced Fibrosis in Renal Tubular Cells via Pyroptosis.

Wen S, Li S, Li L, Fan Q
Biological & pharmaceutical bulletin 2020;43(3):558-564

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Caspase-3 Promotes Diabetic Kidney Disease Through Gasdermin E-Mediated Progression to Secondary Necrosis During Apoptosis.

Wen S, Wang ZH, Zhang CX, Yang Y, Fan QL
Diabetes, metabolic syndrome and obesity : targets and therapy 2020;13:313-323

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Trehalose Alleviates Crystalline Silica-Induced Pulmonary Fibrosis via Activation of the TFEB-Mediated Autophagy-Lysosomal System in Alveolar Macrophages.

He X, Chen S, Li C, Ban J, Wei Y, He Y, Liu F, Chen Y, Chen J
Cells 2020 Jan 4;9(1)

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Nuclear factor-kappa beta signaling is required for transforming growth factor Beta-2 induced ocular hypertension.

Hernandez H, Roberts AL, McDowell CM
Experimental eye research 2020 Feb;191:107920

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Id1 expression in kidney endothelial cells protects against diabetes-induced microvascular injury.

Sharma S, Plotkin M
FEBS open bio 2020 Aug;10(8):1447-1462

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An Improved Method of Maintaining Primary Murine Cardiac Fibroblasts in Two-Dimensional Cell Culture.

Landry NM, Rattan SG, Dixon IMC
Scientific reports 2019 Sep 9;9(1):12889

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Characterization of the extracellular matrix in the chorioallantoic membrane of water buffalo (Bubalus bubalis) in early gestation.

Borghesi J, da Silva Ferreira H, Oliveira Favaron P, Mario LC, de Almeida da Anunciação AR, Oliveira FD, Gonçalves Hayashi R, Caroprezo Morini A, Miglino MA
Reproduction in domestic animals = Zuchthygiene 2019 Oct;54(10):1313-1321

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Tubular GM-CSF Promotes Late MCP-1/CCR2-Mediated Fibrosis and Inflammation after Ischemia/Reperfusion Injury.

Xu L, Sharkey D, Cantley LG
Journal of the American Society of Nephrology : JASN 2019 Oct;30(10):1825-1840

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RNA m(6)A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail.

Lin X, Chai G, Wu Y, Li J, Chen F, Liu J, Luo G, Tauler J, Du J, Lin S, He C, Wang H
Nature communications 2019 May 6;10(1):2065

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Telmisartan attenuates diabetic nephropathy progression by inhibiting the dimerization of angiotensin type-1 receptor and adiponectin receptor-1.

Zha D, Yao T, Bao L, Gao P, Wu X
Life sciences 2019 Mar 15;221:109-120

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Nicotinamide reduces renal interstitial fibrosis by suppressing tubular injury and inflammation.

Zheng M, Cai J, Liu Z, Shu S, Wang Y, Tang C, Dong Z
Journal of cellular and molecular medicine 2019 Jun;23(6):3995-4004

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Mouse placental scaffolds: a three-dimensional environment model for recellularization.

Barreto RS, Romagnolli P, Fratini P, Mess AM, Miglino MA
Journal of tissue engineering 2019 Jan-Dec;10:2041731419867962

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Optimization of Canine Placenta Decellularization: An Alternative Source of Biological Scaffolds for Regenerative Medicine.

Matias GSS, Rigoglio NN, Carreira ACO, Romagnolli P, Barreto RDSN, Mess AM, Miglino MA, Fratini P
Cells, tissues, organs 2018;205(4):217-225

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Activin A induces leiomyoma cell proliferation, extracellular matrix (ECM) accumulation and myofibroblastic transformation of myometrial cells via p38 MAPK.

Bao H, Sin TK, Zhang G
Biochemical and biophysical research communications 2018 Oct 2;504(2):447-453

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Antioxidant and anti-inflammatory activities of berberine attenuate hepatic fibrosis induced by thioacetamide injection in rats.

Eissa LA, Kenawy HI, El-Karef A, Elsherbiny NM, El-Mihi KA
Chemico-biological interactions 2018 Oct 1;294:91-100

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Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog.

Graubner FR, Boos A, Aslan S, Kücükaslan I, Kowalewski MP
Reproduction (Cambridge, England) 2018 May;155(5):403-421

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Fatty acid synthase is required for profibrotic TGF-β signaling.

Jung MY, Kang JH, Hernandez DM, Yin X, Andrianifahanana M, Wang Y, Gonzalez-Guerrico A, Limper AH, Lupu R, Leof EB
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jul;32(7):3803-3815

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Equine lung decellularization: a potential approach for in vitro modeling the role of the extracellular matrix in asthma.

da Palma RK, Fratini P, Schiavo Matias GS, Cereta AD, Guimarães LL, Anunciação ARA, de Oliveira LVF, Farre R, Miglino MA
Journal of tissue engineering 2018 Jan-Dec;9:2041731418810164

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Decellularized bovine cotyledons may serve as biological scaffolds with preserved vascular arrangement.

Barreto RDSN, Romagnolli P, Mess AM, Miglino MA
Journal of tissue engineering and regenerative medicine 2018 Apr;12(4):e1880-e1888

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Recipient Glycemic Micro-environments Govern Therapeutic Effects of Mesenchymal Stem Cell Infusion on Osteopenia.

Sui BD, Hu CH, Zheng CX, Shuai Y, He XN, Gao PP, Zhao P, Li M, Zhang XY, He T, Xuan K, Jin Y
Theranostics 2017;7(5):1225-1244

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Naringin attenuates thioacetamide-induced liver fibrosis in rats through modulation of the PI3K/Akt pathway.

El-Mihi KA, Kenawy HI, El-Karef A, Elsherbiny NM, Eissa LA
Life sciences 2017 Oct 15;187:50-57

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1α,25(OH)₂D₃ Analog, MART-10, Inhibits Neuroendocrine Tumor Cell Metastasis After VEGF-A Stimulation.

Chiang KC, Yeh CN, Pang JS, Hsu JT, Yeh TS, Chen LW, Kuo SF, Takano M, Chen TC, Kittaka A, Hsieh PJ, Juang HH
Anticancer research 2017 Nov;37(11):6215-6221

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Oral Administration of N-Acetyl-seryl-aspartyl-lysyl-proline Ameliorates Kidney Disease in Both Type 1 and Type 2 Diabetic Mice via a Therapeutic Regimen.

Nitta K, Shi S, Nagai T, Kanasaki M, Kitada M, Srivastava SP, Haneda M, Kanasaki K, Koya D
BioMed research international 2016;2016:9172157

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Inositol Hexaphosphate and Inositol Inhibit Colorectal Cancer Metastasis to the Liver in BALB/c Mice.

Fu M, Song Y, Wen Z, Lu X, Cui L
Nutrients 2016 May 12;8(5)

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Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro.

Green CJ, Fraser ST, Day ML
Human reproduction (Oxford, England) 2015 Feb;30(2):284-98

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Chronic carbon monoxide treatment attenuates development of obesity and remodels adipocytes in mice fed a high-fat diet.

Hosick PA, AlAmodi AA, Storm MV, Gousset MU, Pruett BE, Gray W 3rd, Stout J, Stec DE
International journal of obesity (2005) 2014 Jan;38(1):132-9

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Cellular fibronectin expression in human trabecular meshwork and induction by transforming growth factor-β2.

Medina-Ortiz WE, Belmares R, Neubauer S, Wordinger RJ, Clark AF
Investigative ophthalmology & visual science 2013 Oct 17;54(10):6779-88

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The role of peritoneal alternatively activated macrophages in the process of peritoneal fibrosis related to peritoneal dialysis.

Wang J, Jiang ZP, Su N, Fan JJ, Ruan YP, Peng WX, Li YF, Yu XQ
International journal of molecular sciences 2013 May 17;14(5):10369-82

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in NIH 3T3 cell lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: Fibronectin Antibody [NBP1-91258] - Lane view shows a specific band for Fibronectin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fibronectin Antibody [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (http://asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY licence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in human renal cancer using DAB with hematoxylin counterstain.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in mouse kidney tissue section using anti-Fibronectin antibody. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in human oesophageal cancer tissue using anti-Fibronectin antibody. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Feline corneal stromal cells. IHC-Fr and IF (Alexa Fluor488) were performed using NBP1-91258 at 1:400. Image captured by epifluorescent microscopy. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Trabecular meshwork (TM) region of pig eyes. NBP1-91258 Fibronectin antibody was labelled with Alexa Fluor 488 conjugated secondary antibody (Green). DAPI shown as blue. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Staining in mouse carotid artery. Image from a verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Mouse brain cryosections were stained with Fibronectin and anti-rabbit Alexa Fluor 555. Magnification 20x. Image from vrified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.