bs-3543R

antibody from Bioss

Targeting: RELA NFKB3, p65

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

bs-3543R

Provider

Bioss

Proper citation

Bioss Cat#bs-3543R, RRID:AB_10855566

Product name

NFKB p65(Ser276) Polyclonal Antibody

Antibody type

Polyclonal

Description

KLH conjugated synthetic phosphopeptide derived from human NFKBp65 around the phosphorylation site of Ser276

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100ul

Concentration

1ug/ul

Storage

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

References

Submitted references

Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyte.

Jin J, Shi Y, Gong J, Zhao L, Li Y, He Q, Huang H
Stem cell research & therapy 2019 Mar 15;10(1):95

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Pyrazinamide Induced Rat Cholestatic Liver Injury through Inhibition of FXR Regulatory Effect on Bile Acid Synthesis and Transport.

Guo HL, Hassan HM, Zhang Y, Dong SZ, Ding PP, Wang T, Sun LX, Zhang LY, Jiang ZZ
Toxicological sciences : an official journal of the Society of Toxicology 2016 Aug;152(2):417-28

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C11orf95-RELA fusions drive oncogenic NF-κB signalling in ependymoma.

Parker M, Mohankumar KM, Punchihewa C, Weinlich R, Dalton JD, Li Y, Lee R, Tatevossian RG, Phoenix TN, Thiruvenkatam R, White E, Tang B, Orisme W, Gupta K, Rusch M, Chen X, Li Y, Nagahawhatte P, Hedlund E, Finkelstein D, Wu G, Shurtleff S, Easton J, Boggs K, Yergeau D, Vadodaria B, Mulder HL, Becksfort J, Gupta P, Huether R, Ma J, Song G, Gajjar A, Merchant T, Boop F, Smith AA, Ding L, Lu C, Ochoa K, Zhao D, Fulton RS, Fulton LL, Mardis ER, Wilson RK, Downing JR, Green DR, Zhang J, Ellison DW, Gilbertson RJ
Nature 2014 Feb 27;506(7489):451-5

Immunohistochemistry

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded mouse lymphoma labeled with Anti-Phospho-NFKB p65(Ser276)Polyclonal Antibody, Unconjugated (bs-3543R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Sample type

Mouse

Other comments

Lymphoma

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human breast carcinoma labeled with Anti-Phospho-NFKB p65(Ser276)Polyclonal Antibody, Unconjugated(bs-3543R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.

Sample type

Human

Other comments

Breast