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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10338 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10338, RRID:AB_1710359
- Product name
- RELA (phospho S536) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of RELA.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A novel NF-kappaB pathway involving IKKbeta and p65/RelA Ser-536 phosphorylation results in p53 Inhibition in the absence of NF-kappaB transcriptional activity.
Series introduction: the transcription factor NF-kappaB and human disease.
Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kappaB.
Jeong SJ, Pise-Masison CA, Radonovich MF, Park HU, Brady JN
The Journal of biological chemistry 2005 Mar 18;280(11):10326-32
The Journal of biological chemistry 2005 Mar 18;280(11):10326-32
Series introduction: the transcription factor NF-kappaB and human disease.
Baldwin AS Jr
The Journal of clinical investigation 2001 Jan;107(1):3-6
The Journal of clinical investigation 2001 Jan;107(1):3-6
Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kappaB.
Baldwin AS
The Journal of clinical investigation 2001 Feb;107(3):241-6
The Journal of clinical investigation 2001 Feb;107(3):241-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using RELA (phospho S536) polyclonal antibody (Cat # PAB10338) shows detection of RELA phosphorylated at S536.The control blot (left lane) contains 100 ng of purified RELA-GST fusion protein.The band is seen (right lane) when this protein is phosphorylated at S536 by IKKbeta.Personal Communication. J. Brady, NCI, Bethesda, MD.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of RELA (phospho S536) polyclonal antibody (Cat # PAB10338) was used at 5.0 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows moderate positive staining of human kidney distal tubules and collecting ducts.Tissue was formalin-fixed and paraffin embedded.The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
