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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- SM7065P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM7065P, RRID:AB_1005678
- Product name
- anti RELA / NF-kB p65
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide corresponding to amino acids 526-539 of Human NF-kB (p65) protein
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 112A1021
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of NF-kB (p65) using SM7065P at 2 µg/ml in 30 µg of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-Fixed, Paraffin-Embedded Human tonsil using SM7065P at 15 µg/ml.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed with p65 antibody at 5 µg/ml. Human tissue TMA was used for this test.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Intracellular staining of 293 HEK cells using 0.5 ug of SM7065P. Green histogram represents the isotype control, red represents the NF-kB (p65) antibody. Intracellular flow kit was used for this test, and an anti-mouse IgG PE conjugated secondary.
