Explore
Validate
Learn
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN108733 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN108733, RRID:AB_10956271
- Product name
- anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) antibody
- Antibody type
- Monoclonal
- Antigen
- immunisation with tumor cell lines expressing CEACAM6
- Description
- Protein G
- Reactivity
- Human
- Isotype
- IgG
- Antibody clone number
- 9A6
- Vial size
- 100 μg
- Concentration
- 1 mg/mL
- Storage
- short term: 2°C - 8°C, long term: -20°C
- Handling
- Avoid repeated freezing and thawing.
Submitted references Tumor cells in multiple myeloma patients inhibit myeloma-reactive T cells through carcinoembryonic antigen-related cell adhesion molecule-6.
Characterization of adherent-invasive Escherichia coli isolated from pediatric patients with inflammatory bowel disease.
Defining the roles of human carcinoembryonic antigen-related cellular adhesion molecules during neutrophil responses to Neisseria gonorrhoeae.
Adherent-invasive Escherichia coli induce claudin-2 expression and barrier defect in CEABAC10 mice and Crohn's disease patients.
Abnormally expressed ER stress response chaperone Gp96 in CD favours adherent-invasive Escherichia coli invasion.
Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells.
Aberrantly expressed CEACAM6 is involved in the signaling leading to apoptosis of acute lymphoblastic leukemia cells.
Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM.
The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays.
Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism.
Colorectal hyperplasia and dysplasia due to human carcinoembryonic antigen (CEA) family member expression in transgenic mice.
Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1.
Myeloid antigens in childhood lymphoblastic leukemia: clinical data point to regulation of CD66c distinct from other myeloid antigens.
Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site.
Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1; previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear factor-kappaB.
Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells.
Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas.
CEA-related proteins on human urothelial cell lines of different transformation grades.
A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members.
Determination of the specificities of monoclonal antibodies recognizing members of the CEA family using a panel of transfectants.
Multiple organ failure in generalized disseminated tuberculosis.
Effect of NMDA-receptor ligands on neocortical and hippocampal EEG activity of rat brain.
Ovarian relaxin is not essential for dilatation of cervix.
Biochemical characterization and tissue distribution of the Cora antigen, a cell surface glycoprotein differentially expressed on malignant and benign gastrointestinal epithelia.
Witzens-Harig M, Hose D, Jünger S, Pfirschke C, Khandelwal N, Umansky L, Seckinger A, Conrad H, Brackertz B, Rème T, Gueckel B, Meißner T, Hundemer M, Ho AD, Rossi JF, Neben K, Bernhard H, Goldschmidt H, Klein B, Beckhove P
Blood 2013 May 30;121(22):4493-503
Blood 2013 May 30;121(22):4493-503
Characterization of adherent-invasive Escherichia coli isolated from pediatric patients with inflammatory bowel disease.
Negroni A, Costanzo M, Vitali R, Superti F, Bertuccini L, Tinari A, Minelli F, Di Nardo G, Nuti F, Pierdomenico M, Cucchiara S, Stronati L
Inflammatory bowel diseases 2012 May;18(5):913-24
Inflammatory bowel diseases 2012 May;18(5):913-24
Defining the roles of human carcinoembryonic antigen-related cellular adhesion molecules during neutrophil responses to Neisseria gonorrhoeae.
Sarantis H, Gray-Owen SD
Infection and immunity 2012 Jan;80(1):345-58
Infection and immunity 2012 Jan;80(1):345-58
Adherent-invasive Escherichia coli induce claudin-2 expression and barrier defect in CEABAC10 mice and Crohn's disease patients.
Denizot J, Sivignon A, Barreau F, Darcha C, Chan HF, Stanners CP, Hofman P, Darfeuille-Michaud A, Barnich N
Inflammatory bowel diseases 2012 Feb;18(2):294-304
Inflammatory bowel diseases 2012 Feb;18(2):294-304
Abnormally expressed ER stress response chaperone Gp96 in CD favours adherent-invasive Escherichia coli invasion.
Rolhion N, Barnich N, Bringer MA, Glasser AL, Ranc J, Hébuterne X, Hofman P, Darfeuille-Michaud A
Gut 2010 Oct;59(10):1355-62
Gut 2010 Oct;59(10):1355-62
Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells.
Singer BB, Scheffrahn I, Kammerer R, Suttorp N, Ergun S, Slevogt H
PloS one 2010 Jan 18;5(1):e8747
PloS one 2010 Jan 18;5(1):e8747
Aberrantly expressed CEACAM6 is involved in the signaling leading to apoptosis of acute lymphoblastic leukemia cells.
Kanderová V, Hrusák O, Kalina T
Experimental hematology 2010 Aug;38(8):653-660.e1
Experimental hematology 2010 Aug;38(8):653-660.e1
Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM.
Carvalho FA, Barnich N, Sivignon A, Darcha C, Chan CH, Stanners CP, Darfeuille-Michaud A
The Journal of experimental medicine 2009 Sep 28;206(10):2179-89
The Journal of experimental medicine 2009 Sep 28;206(10):2179-89
The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays.
Yue T, Goldstein IJ, Hollingsworth MA, Kaul K, Brand RE, Haab BB
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1697-707
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1697-707
Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism.
Sémiramoth N, Gleizes A, Turbica I, Sandré C, Gorges R, Kansau I, Servin A, Chollet-Martin S
Journal of leukocyte biology 2009 Feb;85(2):310-21
Journal of leukocyte biology 2009 Feb;85(2):310-21
Colorectal hyperplasia and dysplasia due to human carcinoembryonic antigen (CEA) family member expression in transgenic mice.
Chan CH, Camacho-Leal P, Stanners CP
PloS one 2007 Dec 26;2(12):e1353
PloS one 2007 Dec 26;2(12):e1353
Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1.
Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H, Tilki D, Singer BB, Ergün S
Blood 2007 Dec 15;110(13):4223-33
Blood 2007 Dec 15;110(13):4223-33
Myeloid antigens in childhood lymphoblastic leukemia: clinical data point to regulation of CD66c distinct from other myeloid antigens.
Kalina T, Vaskova M, Mejstrikova E, Madzo J, Trka J, Stary J, Hrusak O
BMC cancer 2005 Apr 12;5:38
BMC cancer 2005 Apr 12;5:38
Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site.
Watt SM, Teixeira AM, Zhou GQ, Doyonnas R, Zhang Y, Grunert F, Blumberg RS, Kuroki M, Skubitz KM, Bates PA
Blood 2001 Sep 1;98(5):1469-79
Blood 2001 Sep 1;98(5):1469-79
Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1; previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear factor-kappaB.
Muenzner P, Naumann M, Meyer TF, Gray-Owen SD
The Journal of biological chemistry 2001 Jun 29;276(26):24331-40
The Journal of biological chemistry 2001 Jun 29;276(26):24331-40
Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells.
Muenzner P, Dehio C, Fujiwara T, Achtman M, Meyer TF, Gray-Owen SD
Infection and immunity 2000 Jun;68(6):3601-7
Infection and immunity 2000 Jun;68(6):3601-7
Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas.
Schölzel S, Zimmermann W, Schwarzkopf G, Grunert F, Rogaczewski B, Thompson J
The American journal of pathology 2000 Feb;156(2):595-605
The American journal of pathology 2000 Feb;156(2):595-605
CEA-related proteins on human urothelial cell lines of different transformation grades.
Krop-Watorek A, Laskowska A, Salwa J, Kłopocki AG, Grunert F, Ugorski M
Cancer letters 1999 May 3;139(1):15-22
Cancer letters 1999 May 3;139(1):15-22
A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members.
Thompson J, Mössinger S, Reichardt V, Engels U, Beauchemin N, Kommoss F, von Kleist S, Zimmermann W
International journal of cancer. Journal international du cancer 1993 Sep 9;55(2):311-9
International journal of cancer. Journal international du cancer 1993 Sep 9;55(2):311-9
Determination of the specificities of monoclonal antibodies recognizing members of the CEA family using a panel of transfectants.
Daniel S, Nagel G, Johnson JP, Lobo FM, Hirn M, Jantscheff P, Kuroki M, von Kleist S, Grunert F
International journal of cancer. Journal international du cancer 1993 Sep 9;55(2):303-10
International journal of cancer. Journal international du cancer 1993 Sep 9;55(2):303-10
Multiple organ failure in generalized disseminated tuberculosis.
Sydow M, Schauer A, Crozier TA, Burchardi H
Respiratory medicine 1992 Nov;86(6):517-9
Respiratory medicine 1992 Nov;86(6):517-9
Effect of NMDA-receptor ligands on neocortical and hippocampal EEG activity of rat brain.
Sharma AC, Kulkarni SK, Nayar U
Indian journal of experimental biology 1991 Aug;29(8):744-50
Indian journal of experimental biology 1991 Aug;29(8):744-50
Ovarian relaxin is not essential for dilatation of cervix.
Eddie LW, Cameron IT, Leeton JF, Healy DL, Renou P
Lancet 1990 Jul 28;336(8709):243
Lancet 1990 Jul 28;336(8709):243
Biochemical characterization and tissue distribution of the Cora antigen, a cell surface glycoprotein differentially expressed on malignant and benign gastrointestinal epithelia.
Göttlinger HG, Lobo FM, Grimm TW, Riethmüller G, Johnson JP
Cancer research 1988 Apr 15;48(8):2198-203
Cancer research 1988 Apr 15;48(8):2198-203
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6, 7 or 8. Recognition of CEACAM3 was tested on stably transfected HeLa cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibo-dies known to recognize the corresponding proteins (CEACAM1, 3, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3 (green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified 9A6 was tested on all CEACAM transfectants. A posi-tive signal was only obtained with CEACAM6-expressing cells (red curves).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using 9A6. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM6 (red curve) or an irrelevant protein (control transfectant). Binding of 9A6 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM6 transfected cells.
