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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 649704 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#649704, RRID:AB_2562425
- Product name
- Alexa Fluor® 647 anti-Pax-5
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- 1H9
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 4° C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- C57BL/6 mouse splenocytes were surface stained with mouse CD3, then fixed, and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set (Cat# 422601), then intracellularly stained with Pax-5 (clone 1H9) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
- Conjugate
- Red dye
