Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-00258-100ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Pax5/BSAP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pax5/BSAP Antibody (1H9) [NBP2-00258] - Daudi cell lysates prepared under reducing conditions were resolved by SDS-PAGE then immunoblotted with 2 ug/ml of Anti-Human/Mouse Pax5 Purified. Bands were visualized using Anti-Rat IgG HRP.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Pax5/BSAP Antibody (1H9) [NBP2-00258] - Analysis using the PE conjugate of NBP2-00258. Surface staining of C57BL/6 splenocytes with Anti-Mouse CD23 PE-Cy7 and Anti-Mouse CD21/CD35 FITC followed by intracellular staining using the Foxp3 Staining Buffer Set and 0.125 ug of Anti-Human/Mouse Pax5 PE. Histogram depicts cell populations based on surface stains comprising the marginal zone cells (CD21(high)CD23-) (blue histogram) or T1 cells (CD21-CD23-) (green histogram).