Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA1-39420 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAX5 Monoclonal Antibody (SP34)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP34
- Vial size
- 1 mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A 2-Step Laemmli and Antigen Retrieval Method Improves Immunodetection.
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
Composite lymphoma in the anterior mediastinum: a case report and review of the literature.
Scalia CR, Gendusa R, Cattoretti G
Applied immunohistochemistry & molecular morphology : AIMM 2016 Jul;24(6):436-46
Applied immunohistochemistry & molecular morphology : AIMM 2016 Jul;24(6):436-46
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
Sauter JL, Ambaye AB, Mount SL
Diagnostic cytopathology 2015 Sep;43(9):688-95
Diagnostic cytopathology 2015 Sep;43(9):688-95
Composite lymphoma in the anterior mediastinum: a case report and review of the literature.
Yu G, Kong L, Qu G, Zhang Q, Wang W, Jiang L
Diagnostic pathology 2011 Jul 6;6:60
Diagnostic pathology 2011 Jul 6;6:60
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PAX-5 using anti-PAX-5 Monoclonal Antibody (Product # MA5-16389) in Tonsil Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PAX5 using a monoclonal antibody (Product # MA1-39420).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on formalin-fixed paraffin-embedded canine lymph node tissue sections. Antigen retrieval was performed by heating tissues in 10mM sodium citrate buffer, pH 9.0 for 20 minutes. Tissues were blocked in 10% normal goat serum containing 2X casein for 30 minutes at room temperature and probed with a PAX5 monoclonal antibody, clone SP34 (Product # MA1-39420) for 1.5 hours at 37°C. Detection was performed using a biotinylated goat anti-rabbit IgG secondary antibody description at a dilution of 1:200 for 30 minutes at room temperature followed by streptavidin-HRP for 20 minutes at room temperature and a chromogenic substrate. Tissues were visualized by light microscopy. Data courtesy of the Innovators Program.
