TA500590

antibody from OriGene

Targeting: CRYAB CRYA2, HSPB5

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500590

Provider

OriGene

Proper citation

OriGene Cat#TA500590, RRID:AB_11140727

Product name

CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI1G1 (formerly 1G1)

Antibody type

Monoclonal

Description

CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI1G1 (formerly 1G1)

Host

Mouse

Conjugate

Unconjugated

Epitope

CRYAB

Isotype

IgG

Antibody clone number

OTI1G1

Vial size

100 µl

Concentration

1.00mg/ml

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-CRYAB mouse monoclonal antibody (TA500590) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB(RC202718).

Validation comment

IF

Immunoprecipitation

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunoprecipitation(IP) of CRYAB by using TrueMab monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Validation comment

IP

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-CRYAB antibody(TA500590),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC202718 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CRYAB antibody(TA500590), and then analyzed by flow cytometry.

Validation comment

FC