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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- MA5-15383 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRYAB Monoclonal Antibody (1D11C6E6)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15383 targets CRYAB in IHC and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 1D11C6E6
- Concentration
- Conc. Not Determined
Submitted references Presence of melanopsin in human crystalline lens epithelial cells and its role in melatonin synthesis.
Alkozi HA, Wang X, Perez de Lara MJ, Pintor J
Experimental eye research 2017 Jan;154:168-176
Experimental eye research 2017 Jan;154:168-176
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CRYAB was performed by loading 5 µg of human brain tumor tissue lysates per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% milk in TBST for 20 minutes. The membrane was probed with a CRYAB monoclonal antibody (Product # MA5-15383) at a dilution of 1:500 overnight at 4°C, washed in TBS + 0.1% Tween-20, and probed with an HRP-conjugated anti-mouse IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature. Detection was performed using a chemiluminescent substrate. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CRYAB using CRYAB monoclonal antibody (Product # MA5-15383) in HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CRYAB cDNA (2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain hippocampus (A), lung cancer (B), brain tumor (C), breast cance (D) using CRYAB monoclonal antibody (Product # MA5-15383) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human brain tumor tissue sections. To expose target antigens, heat-induced epitope retrieval was performed by heating tissues at 120°C for 5 minutes in sodium citrate buffer pH 6.0. Tissues were blocked in 3% hydrogen peroxide for 5 minutes at room temperature and probed with a CRYAB monoclonal antibody (Product # MA5-15383) at a dilution of 1:500 for 80 minutes at room temperature. Tissues were washed extensively with TBST. Detection was performed using an HRP-conjugated anti-mouse IgG secondary antibody and colorimetric substrate. Tissues were counterstained with hematoxylin and visualized by light microscopy. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissues using CRYAB monoclonal antibody (Product # MA5-15383).
