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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27419 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD40 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: K562, THP-1.
- Concentration
- 0.77 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD40 Polyclonal Antibody (Product # PA5-27419). Sample (30 µg of whole cell lysate). Lane A: THP-1 . 15% SDS PAGE. CD40 Polyclonal Antibody (Product # PA5-27419) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CD40 was performed in THP-1 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CD40 Polyclonal Antibody (Product # PA5-27419) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using CD40 (Product # PA5-27419) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
