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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA324374 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal USF1 Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal USF1 Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- USF1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- USF1 Antibody (Center) (Cat. #TA324374) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the USF1 antibody detected the USF1 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of SK-BR-3 cell stained with USF1 Antibody (Center)(Cat#TA324374). SK-BR-3 cells were incubated with USF1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .USF1 immunoreactivity is localized to nucleus significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- USF1 Antibody (Center) (Cat. #TA324374)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- USF1 Antibody (Center) (Cat. #TA324374) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
