Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00006777-M04 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006777-M04, RRID:AB_733248
- Product name
- STAT5B monoclonal antibody (M04), clone 1C2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant STAT5B.
- Antigen sequence
MAVWIQAQQLQGEALHQMQALYGQHFPIEVRHYLS
QWIESQAWDSVDLDNPQENIKATQLLEGLVQELQK
KAEHQVGEDGFLLKIKLGHYATQLQNTYDRCPMEL
VRCIRHILYNEQRLVREANNGSSPAGSLADAMSQK
HLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQ
YQESLRIQAQFGPLAQLSPQERLSRETALQQKQVS
LEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQT
IILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCE
KLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAE
VNATITDIISALVTSTFIIEKQPPQVLKTQTKFAA
TVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKN
ENTRK- Isotype
- IgG
- Antibody clone number
- 1C2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- STAT5B monoclonal antibody (M04), clone 1C2 Western Blot analysis of STAT5B expression in K-562 ( Cat # L009V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of STAT5B expression in transfected 293T cell line by STAT5B monoclonal antibody (M04), clone 1C2.Lane 1: STAT5B transfected lysate (Predicted MW: 44.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged STAT5B is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of STAT5B transfected lysate using anti-STAT5B monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with STAT5B monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol