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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF152 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-13R alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IL-13 R alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinat human (rh) IL-13 R alpha 2, recombinant mouse IL-13 R alpha 1, rhIL-5 R alpha , rhIL-5 R beta , rhIL-4 R, and rhIL-9 R is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Optimization of IL13Rα2-Targeted Chimeric Antigen Receptor T Cells for Improved Anti-tumor Efficacy against Glioblastoma.
Expression of surfactant protein D in airways of asthmatics and interleukin-13 modulation of surfactant protein D in human models of airway epithelium.
IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells.
Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1β-stimulated production of chemokines and matrix-degrading enzymes in vitro.
Obligatory role for interleukin-13 in obstructive lesion development in airway allografts.
Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
Differential effect of IL-4 and IL-13 on the expression of recombination-activating genes in mature B cells from human peripheral blood.
Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.
Brown CE, Aguilar B, Starr R, Yang X, Chang WC, Weng L, Chang B, Sarkissian A, Brito A, Sanchez JF, Ostberg JR, D'Apuzzo M, Badie B, Barish ME, Forman SJ
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Jan 3;26(1):31-44
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Jan 3;26(1):31-44
Expression of surfactant protein D in airways of asthmatics and interleukin-13 modulation of surfactant protein D in human models of airway epithelium.
Xu J, Singhera GK, Dorscheid DR
Respiratory research 2015 Feb 15;16:26
Respiratory research 2015 Feb 15;16:26
IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells.
Reece P, Gauvreau GM, Sehmi R, Denburg JA
PloS one 2014;9(6):e100734
PloS one 2014;9(6):e100734
Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1β-stimulated production of chemokines and matrix-degrading enzymes in vitro.
Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzì RM, Meliconi R
PloS one 2014;9(5):e96925
PloS one 2014;9(5):e96925
Obligatory role for interleukin-13 in obstructive lesion development in airway allografts.
Lama VN, Harada H, Badri LN, Flint A, Hogaboam CM, McKenzie A, Martinez FJ, Toews GB, Moore BB, Pinsky DJ
The American journal of pathology 2006 Jul;169(1):47-60
The American journal of pathology 2006 Jul;169(1):47-60
Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M
International journal of oncology 2004 Sep;25(3):605-13
International journal of oncology 2004 Sep;25(3):605-13
Differential effect of IL-4 and IL-13 on the expression of recombination-activating genes in mature B cells from human peripheral blood.
Kajiwara K, Shinazawa M, Morishima H, Yanagihara Y
Cellular immunology 2004 Feb;227(2):121-8
Cellular immunology 2004 Feb;227(2):121-8
Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.
Zhou Y, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ
The American journal of pathology 2002 Apr;160(4):1405-23
The American journal of pathology 2002 Apr;160(4):1405-23
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-13 R alpha 1 in Human Skin. IL-13 R alpha 1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL-13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of IL-13 R alpha 1 in Human Granulocytes by Flow Cytometry. Human whole blood granulocytes were stained with Goat Anti-Human IL-13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-13 and Neutralization by Human IL-13 R alpha 1 Antibody. Recombinant Human IL-13 (Catalog # 213-ILB) stimulates prolif-eration in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152). The ND50 is typically 10-30 µg/mL.
